| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF/ICC, ELISA | 57 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:5000-20000 IHC-P=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | CYP3A4 Mouse Monoclonal Antibody detects endogenous levels of CYP3A4 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
CYP3A4 is a member of the cytochrome P450 superfamily of enzymes. CYP3A4 plays a crucial role in drug metabolism, as CYP3A4 is responsible for the biotransformation of over 50% of clinically used medications, making CYP3A4 essential for pharmacokinetics and personalized medicine. Located predominantly in the endoplasmic reticulum of liver cells and upper intestinal enterocytes, CYP3A4′s expression can be induced by glucocorticoids and other pharmacological agents, which can significantly affect drug clearance and efficacy. The CYP3A gene cluster, located at the 7q22.1 locus, includes CYP3A4 along with CYP3A5, CYP3A7, and CYP3A43, as well as two pseudogenes, highlighting the complexity and importance of this enzyme family in drug metabolism and the synthesis of cholesterol, steroids, and other lipids. |
| Product Image Gallery | |
 Western blot analysis of extracts on HepG2, Mouse liver cells, using CYP3A4 Mouse Monoclonal Antibody (EAB22111) at 1:10000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
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