| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IHC-P, IF/ICC, FC | 37 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-200 IF/ICC=1:50-200 FC=1:10-100 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | PP1β Rabbit Monoclonal Antibody detects endogenous levels of PP1β protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
PP1β also known as PP1beta, PPP1CB is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1β is a pivotal catalytic subunit within the Protein Phosphatase 1 (PP1) family, characterized by its conserved catalytic domain essential for serine/threonine phosphatase activity and a unique C-terminal region that mediates interactions with regulatory and targeting proteins. Post-translational modifications (PTMs) of PP1β, such as phosphorylation, methylation, and ubiquitination, play critical roles in modulating activity, stability, and subcellular localization, thereby fine-tuning involvement in key cellular processes including cell cycle regulation, metabolism, and apoptosis. Phosphorylation of PP1β can alter enzymatic activity and influence binding affinity to regulatory subunits, thereby impacting ability to dephosphorylate specific substrates. Methylation and ubiquitination further regulate PP1β by affecting interactions with other proteins and degradation via the proteasome pathway, respectively. |
| Product Image Gallery | |
 Western blot analysis of extracts on A431, PC12, Jurkat, NIH/3T3, Mouse brain cells, using PP1β Rabbit Monoclonal Antibody (EAB22048) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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