| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IHC-P, IF/ICC, ELISA | 45 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IP=1:10-100 IHC-P=1:50-200 IF/ICC=1:50-200 FC=1:10-100 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | PSMC5/TRIP1 Rabbit Monoclonal Antibody detects endogenous levels of PSMC5/TRIP1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
PSMC5 also known as SUG1, TRIP1 is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. PSMC5 plays a crucial role as an ATPase subunit within the 26S proteasome complex, which is essential for the regulated degradation of ubiquitinated proteins. PSMC5 is localized in both the cytoplasm and nucleus, where PSMC5 is part of the 19S regulatory particle that confers substrate specificity and facilitates the ATP-dependent degradation of proteins, particularly those misfolded or damaged in the endoplasmic reticulum. PSMC5′s importance extends to involvement in the assembly of the 26S proteasome, which has been shown to be dependent on phosphorylation by specific protein kinases. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, Jurkat, SH-SY5Y cells, using PSMC5/TRIP1 Rabbit Monoclonal Antibody (EAB22046) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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