| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG2a | Monoclonal | WB, IHC-P, IF/ICC, FC, ELISA | 85 | Human, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:100-500 IF/ICC=1:100-500 FC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | p95/NBS1 Mouse Monoclonal Antibody detects endogenous levels of p95/NBS1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
p95/NBS1 also known as NBS, NBS1, P95, NBN, Nibrin is a component of the MRE11/RAD50/NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity, particularly in repairing double-strand breaks, which are among the most lethal forms of DNA damage. p95/NBS1 functions as part of a complex with Mre11 and Rad50, forming the MRE11/RAD50/NBS1 (MRN) complex that is essential for recognizing and processing DNA breaks. p95/NBS1′s importance is underscored by its absence in Nijmegen breakage syndrome (NBS) patients, who exhibit increased sensitivity to ionizing radiation and predisposition to cancer due to defective DNA repair mechanisms. |
| Product Image Gallery | |
 Western blot analysis of extracts on A549, PC12, Jurkat cells, using p95/NBS1 Mouse Monoclonal Antibody (EAB21983) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
Predicted band size: 85 kDa; Observed band size: 95 kDa |
