| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IHC-P, IF/ICC | 43 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-200 IF/ICC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | NCK2 Rabbit Monoclonal Antibody detects endogenous levels of NCK2 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
NCK2 also known as NCKbeta, GRB4 is a member of the NCK family of adaptor proteins. NCK2 plays a crucial role in cellular signaling as a member of the NCK family of SH2/SH3 adaptor proteins, which also includes NCK1. These proteins are essential for coupling tyrosine kinase signaling pathways, such as those activated by epidermal growth factor (EGF) and platelet-derived growth factor (PDGF), to downstream signaling proteins. NCK2 binds to human PDGFR-β at tyrosine 1009, and overexpression has been shown to inhibit PDGF-induced membrane ruffling and lamellipod formation, thereby regulating cell motility and morphology. Additionally, the SH2 domain of NCK2 has been implicated in the inhibition of EGF- and PDGF-induced DNA synthesis, highlighting a role in controlling cell proliferation. |
| Product Image Gallery | |
 Western blot analysis of extracts on MCF7, Mouse brain cells, using NCK2 Rabbit Monoclonal Antibody (EAB22152) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
|
