| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG2b | Monoclonal | WB, IHC-P, IF/ICC, FC, ELISA | 54 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:5000-20000 IHC-P=1:500-2000 IF/ICC=1:200-1000 FC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Nrf1 Mouse Monoclonal Antibody detects endogenous levels of Nrf1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Nrf1(Nuclear respiratory factor 1) is a member of the Cap ′n′ collar (CNC) family of bZIP transcription factors. Nrf1 plays a crucial role in cellular defense mechanisms by regulating the expression of genes involved in the antioxidant response and detoxification processes. Nrf1 is primarily located in the nucleus, where translocation occurs in response to oxidative stress and xenobiotic exposure, allowing activation of target genes such as those encoding detoxifying enzymes. Nrf1′s ability to heterodimerize with other proteins, including MafK and members of the Jun family, enhances transcriptional activity, making Nrf1 essential for maintaining cellular homeostasis and protecting against oxidative damage. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, HEK293, NIH/3T3 cells, using Nrf1 Mouse Monoclonal Antibody (EAB22031) at 1:10000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
Predicted band size: 54 kDa; Observed band size: 70 kDa |
