| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IF/ICC, ELISA | 22 | Human |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | MGMT Mouse Monoclonal Antibody detects endogenous levels of MGMT protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
MGMT also known as AGT is a vital DNA repair enzyme that removes mutagenic and cytotoxic alkyl groups from the O6 position of guanine in DNA. Such alkylation lesions can lead to mispairing during DNA replication, resulting in mutations and potentially carcinogenesis. MGMT is predominantly located in the cell nucleus, where direct access to genomic DNA allows for efficient repair of DNA damage before cell division occurs. Nuclear localization ensures maintenance of genomic stability and prevents propagation of DNA errors to daughter cells. High levels of MGMT can confer resistance to alkylating chemotherapeutic agents, making MGMT expression significant in cancer research and therapy development. |
| Product Image Gallery | |
 Western blot analysis of extracts on MCF7, Jurkat, HepG2, PC3 cells, using MGMT Mouse Monoclonal Antibody (EAB22039) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
 Immunofluorescence analysis of Hela cell, using MGMT Mouse Monoclonal Antibody (EAB22039)(green) at 1:200 dilution.
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