| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG2a | Monoclonal | WB, IHC-P, IF/ICC, ELISA | 84 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IHC-P=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | MFN1/Mitofusin-1 Mouse Monoclonal Antibody detects endogenous levels of MFN1/Mitofusin-1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
MFN1(Mitofusin 1) is a mediator of mitochondrial fusion. MFN1 plays a crucial role in mitochondrial dynamics, specifically in mitochondrial fusion, which is essential for maintaining mitochondrial function and cellular energy metabolism. MFN1 localizes to the outer mitochondrial membrane and forms high molecular weight protein complexes that facilitate mitochondrial fusion, a process vital for cellular health and stress adaptation. MFN1 contains a GTPase domain critical for function, with purified recombinant MFN1 showing approximately eightfold higher GTPase activity compared to MFN2. This activity difference may influence distinct roles of MFN1 and MFN2 in cellular processes, including varying expression levels across tissues, with MFN1 transcripts being particularly elevated in heart tissue. |
| Product Image Gallery | |
 Western blot analysis of extracts on K562, HepG2, Jurkat, NIH/3T3 cells, using MFN1/Mitofusin-1 Mouse Monoclonal Antibody (EAB22139) at 1:3000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
|
