| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG2b | Monoclonal | WB, IHC-P, IF/ICC, ELISA | 69 | Human |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | PTGS2/Cox2 Mouse Monoclonal Antibody detects endogenous levels of PTGS2/Cox2 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Cox2 also known as PTGS2 is an inducible enzyme that plays a pivotal role in the conversion of arachidonic acid to prostaglandins, which are lipid mediators involved in inflammation, pain, and fever. Unlike its constitutively expressed isoform Cox1, Cox2 is minimally expressed under normal physiological conditions but is significantly upregulated in response to inflammatory stimuli, cytokines, growth factors, and tumor promoters. This upregulation leads to increased production of prostaglandins, contributing to the inflammatory response and pain perception. Overexpression of Cox2 has been implicated in various pathological conditions, including chronic inflammation, arthritis, and the progression of certain cancers such as colorectal, breast, and lung cancer. Cox2 is a key therapeutic target for nonsteroidal anti-inflammatory drugs (NSAIDs) and selective Cox2 inhibitors (coxibs), which aim to reduce inflammation and pain by inhibiting prostaglandin synthesis. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, A549 cells, using PTGS2/Cox2 Mouse Monoclonal Antibody (EAB22133) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
|
