| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IHC-P, IF/ICC | 29 | Human, Bovine |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IP=1:10-100 IHC-P=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | TFAM/mtTFA Rabbit Monoclonal Antibody detects endogenous levels of TFAM/mtTFA protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
mtTFA(mitochondrial transcription factor A) also known as TFAM, TCF6 is a nuclear-encoded protein imported into mitochondria, where mtTFA plays a crucial role in mitochondrial biogenesis by regulating the replication and transcription of mitochondrial DNA (mtDNA). mtTFA function is essential for maintaining mtDNA copy number and integrity, which is vital for proper mitochondrial function and energy production in cells. Alterations in mtTFA expression have been linked to mitochondrial disorders characterized by symptoms like ocular myopathy, exercise intolerance, and muscle wasting, underscoring mtTFA importance in human health. During spermatogenesis in mice and humans, a reduction in mtTFA protein levels leads to decreased mtDNA copy number, which may help prevent paternal mtDNA transmission. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, K562 cells, using TFAM/mtTFA Rabbit Monoclonal Antibody
(EAB21978) at 1:2000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 29 kDa; Observed band size: 24 kDa |
