| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IHC-P, IF/ICC, FC | 24 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IP=1:10-100 IHC-P=1:50-200 IF/ICC=1:50-200 FC=1:10-100 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | MAD2L2 Rabbit Monoclonal Antibody detects endogenous levels of MAD2L2 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
MAD2L2 also konwn as MAD2B is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L2 plays a vital role in the regulation of the mitotic spindle assembly checkpoint, ensuring that cells do not proceed to anaphase until all chromosomes are properly attached to the spindle apparatus. This function is crucial for maintaining genomic stability, as errors in chromosome segregation can lead to aneuploidy and contribute to tumorigenesis. MAD2L2 is localized to unattached kinetochores during pro-metaphase, where MAD2L2 acts as a sensor for spindle attachment; upon proper attachment, MAD2L2 disassociates, allowing the cell cycle to progress. Furthermore, MAD2L2 has been shown to interact with various proteins involved in cell cycle regulation, including the Insulin receptor, which suggests MAD2L2′s involvement in mediating specific signaling pathways that influence cell proliferation and survival. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, Jurkat, MCF7 cells, using MAD2L2 Rabbit Monoclonal Antibody (EAB22062) at 1:500 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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