| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IHC-P, IF/ICC | 102 | Human, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-200 IF/ICC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | DNA Ligase I/LIG1 Rabbit Monoclonal Antibody detects endogenous levels of DNA Ligase I/LIG1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
DNA Ligase I also known as LIG1 is a member of the ATP-dependent DNA ligase protein family. DNA Ligase I plays a vital role in DNA replication and repair by joining Okazaki fragments during lagging strand synthesis and sealing nicks in DNA during excision repair processes. DNA Ligase I is predominantly located in the nucleus, where DNA Ligase I activity is essential for proper DNA repair pathways, ensuring efficient repair of DNA breaks to prevent genomic instability. Notably, elevated levels of DNA Ligase I have been observed in various human tumors compared to benign tissues, highlighting DNA Ligase I potential as a biomarker for cancer progression and a target for therapeutic intervention. Additionally, DNA Ligase I antisense oligonucleotides have been shown to reduce tumor cell proliferation, suggesting a significant role in cancer biology. |
| Product Image Gallery | |
 Western blot analysis of extracts on Jurkat cells, using DNA Ligase I/LIG1 Rabbit Monoclonal Antibody (EAB21984) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 102 kDa; Observed band size: 140 kDa |
