| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP | 121 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IP=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | JMJD2A Rabbit Monoclonal Antibody detects endogenous levels of JMJD2A protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
JMJD2A also known as KDM4A is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein containing a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains. JMJD2A plays a crucial role in gene expression regulation by specifically demethylating lysine residues 9 and 36 on histone H3, essential for proper interpretation of the histone code. JMJD2A′s ability to demethylate tri-methylated H3 Lys 9 and H3 Lys 36, while leaving other residues untouched, highlights JMJD2A′s specificity and importance in chromatin remodeling and transcriptional regulation. JMJD2A generates formaldehyde and succinate as byproducts of demethylation activity, which can have further implications in cellular metabolism and signaling pathways. |
| Product Image Gallery | |
 Western blot analysis of extracts on NIH/3T3, 293T, Rat brain cells, using JMJD2A Rabbit Monoclonal Antibody (EAB22774) at 1:5000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 121 kDa; Observed band size: 150 kDa |
