| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IHC-P, IF/ICC | 48 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IP=1:10-100 IHC-P=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | IKKγ Rabbit Monoclonal Antibody detects endogenous levels of IKKγ protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
IKKγ also known as IKKgamma, NEMO, IKBKG plays a pivotal role in the NF-κB signaling pathway, acting as an essential regulatory subunit of the IKK complex, which is crucial for the phosphorylation and degradation of IκB proteins. This process allows the transcription factor NF-κB to translocate to the nucleus and activate genes involved in immune responses, inflammation, and cell survival, making IKKγ a critical target for studying these fundamental biological mechanisms. The functionality of IKKγ is vital for understanding how cells respond to proinflammatory cytokines and stress signals, highlighting its importance in various physiological and pathological contexts. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, Jurkat, MCF7, HepG2 cells, using IKKγ Rabbit Monoclonal Antibody (EAB22025) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 48 kDa; Observed band size: 43-60 kDa |
