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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, ELISA | 24 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:2000-10000 IHC-P=1:200-1000 |
|
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | HMGB2 Mouse Monoclonal Antibody detects endogenous levels of HMGB2 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
HMGB2 also known as HMG2 is a member of the non-histone chromosomal high mobility group protein family. HMGB2 play a crucial role in regulating gene expression by influencing chromatin structure. HMGB2 binds to DNA and induces significant conformational changes, such as bending and unwinding of the DNA helix, which are essential for transcription factor accessibility to target genes. This dynamic interaction enables proper functioning of various cellular processes, including transcriptional activation, where HMGB2 collaborates with other transcription factors like Oct-2 and members of the NFκB family. Post-translational modifications, such as phosphorylation, enhance HMGB2 transcriptional activity, highlighting their importance in cellular signaling pathways. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, MCF7, Jurkat, HEK293 cells, using HMGB2 Mouse Monoclonal Antibody (EAB22583) at 1:5000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
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