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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IHC-P, IF/ICC | 25 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:1000-5000 IP=1:10-100 IHC-P=1:100-500 IF/ICC=1:100-500 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | HPRT Rabbit Monoclonal Antibody detects endogenous levels of HPRT protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
HPRT(Hypoxanthine phosphoribosyltransferase) is a crucial cytoplasmic protein composed and belongs to the purine/pyrimidine phosphoribosyltransferase family. HPRT plays a vital role in purine metabolism as a purine salvage enzyme. HPRT catalyzes the conversion of hypoxanthine and guanine into their respective mononucleotides, inosine monophosphate and guanosine monophosphate, thereby facilitating the recycling of purines, which are essential for DNA and RNA synthesis. HPRT exists as a homotetramer and requires two magnesium ions as cofactors for enzymatic activity. Deficiencies in HPRT can lead to serious metabolic disorders, including gout and Lesch-Nyhan syndrome, highlighting HPRT′s importance in maintaining purine homeostasis. |
| Product Image Gallery | |
 Western blot analysis of extracts on K562, HEK293, Mouse brain cells, using HPRT Rabbit Monoclonal Antibody (EAB22050) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemistry of paraffin-embedded Human tonsil tissue, using HPRT Rabbit Polyclonal Antibody (EAB22050) at 1:200 dilution.
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