| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IHC-P, IF/ICC | 24 | Human |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-200 IF/ICC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Geminin Rabbit Monoclonal Antibody detects endogenous levels of Geminin protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Geminin also known as GMNN is a protein that plays a crucial role in regulating DNA replication initiation during the cell cycle, ensuring DNA replicates only once per cycle to maintain genomic stability. Geminin expression levels are tightly regulated, peaking during the S, G2, and M phases of the cell cycle, while remaining lowest during the G1 phase. This fluctuation is essential, as geminin degradation during mitosis, mediated by the anaphase-promoting complex (APC), allows for the re-establishment of the replication machinery in the subsequent cell cycle. Precise control of geminin levels is critical for cellular function, as dysregulation can lead to genomic instability and contribute to oncogenesis. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, MCF7, Jurkat cells, using Geminin Rabbit Monoclonal Antibody (EAB21960) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 24 kDa; Observed band size: 30 kDa |
