| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IF/ICC, ELISA | 148 | Human |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | HER3/ErbB3 Mouse Monoclonal Antibody detects endogenous levels of HER3/ErbB3 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
ErbB3 also known as HER3 is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. ErbB3 is predominantly located on the cell membrane where ErbB3 plays a pivotal role in cellular signaling pathways that regulate cell growth, differentiation, and survival. Structurally, ErbB3 consists of an extracellular ligand-binding domain, a single transmembrane region, and an intracellular domain rich in tyrosine residues essential for downstream signaling. Post-translational modifications, particularly phosphorylation of specific tyrosine residues within the cytoplasmic domain, are crucial for ErbB3′s function. These phosphorylations create binding sites for signaling molecules such as the p85 subunit of phosphoinositide 3-kinase (PI3K), thereby activating the PI3K/Akt pathway, which is vital for promoting oncogenic processes including tumor growth and resistance to apoptosis. |
| Product Image Gallery | |
 Western blot analysis of extracts on HEK293 cells, using HER3/ErbB3 Mouse Monoclonal Antibody (EAB22011) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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