| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IF/ICC, FC, ELISA | 69 | Human, Mouse, Monkey |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IF/ICC=1:100-500 FC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Ezrin Mouse Monoclonal Antibody detects endogenous levels of Ezrin protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Ezrin also known as EZR plays a crucial role in linking the cytoskeleton to the plasma membrane, which is essential for maintaining cell shape and facilitating cellular signaling. Ezrin is primarily located just beneath the plasma membrane, where Ezrin interacts with other cytoskeletal and membrane proteins, thereby influencing processes such as cell adhesion, migration, and morphology. Notably, Ezrin acts as a major cytoplasmic substrate for various protein-tyrosine kinases, including the epidermal growth factor receptor, and functions as an A-kinase anchoring protein, designated AKAP78. Ezrin′s interaction with Moesin and Radixin, which share over 70% homology, highlights the importance of this protein family in cellular dynamics, as these proteins exhibit distinct receptor-specific patterns of phosphorylation despite their similarities. This specificity is vital for the regulation of various signaling pathways, making Ezrin a key player in cellular responses to external stimuli. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, A431, HEK293, NIH/3T3 cells, using Ezrin Mouse Monoclonal Antibody (EAB22005) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
 Immunofluorescence analysis of Hela cell, using Ezrin Mouse Monoclonal Antibody (EAB22005)(green) at 1:200 dilution.
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