| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF/ICC, FC, ELISA | 66 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:100-500 IF/ICC=1:100-500 FC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Estrogen receptor α/ERα Mouse Monoclonal Antibody detects endogenous levels of Estrogen receptor α/ERα protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
ERα(Estrogen Receptor α) also known as ESR1 is a ligand-activated transcription factor belonging to the steroid/thyroid hormone receptor superfamily. ERα plays a critical role in regulating the normal function of reproductive tissues. Post-translational modifications (PTMs) of ERα, such as phosphorylation, ubiquitination, sumoylation, acetylation, and methylation, are crucial for modulating activity, stability, cellular localization, and interactions with co-regulatory proteins. For instance, phosphorylation at specific serine residues can enhance or repress ERα′s transcriptional activity, influencing gene expression involved in cell proliferation and differentiation. Ubiquitination targets ERα for proteasomal degradation, thereby controlling receptor levels within the cell and affecting hormonal responsiveness. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, SKBR3, MCF7, Raji cells, using Estrogen receptor α/ERα Mouse Monoclonal Antibody (EAB22611) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
 Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue, using Estrogen receptor α/ERα Mouse Monoclonal Antibody (EAB22611) at 1:400 dilution
 Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue, using Estrogen receptor α/ERα Mouse Monoclonal Antibody (EAB22611) at 1:200 dilution.
 Immunofluorescence analysis of Hela cell, using Estrogen receptor α/ERα Mouse Monoclonal Antibody (EAB22611)(blue) at 1:200 dilution.
 Flow cytometric analysis of Hela cell, using Estrogen receptor α/ERα Mouse Monoclonal Antibody (EAB22611) at 1:200 dilution.
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