| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, ELISA | 45 | Human, Mouse |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | DnaJA4 Rabbit Polyclonal Antibody detects endogenous levels of DnaJA4 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
DnaJA4 is a member of the DnaJ family and is characterized by diverse cellular localization and functions, primarily defined by the presence of the J domain. DnaJA4 plays a crucial role in regulating the cholesterol biosynthesis pathway, acting as a SREBP-regulated chaperone. DnaJA4 is essential for maintaining cellular homeostasis, particularly in lipid metabolism, as DnaJA4 assists in proper folding and functioning of other proteins involved in cholesterol synthesis. DnaJA4 interaction with HSP 70 enhances ATP hydrolysis, which is vital for chaperone activity in preventing protein aggregation and facilitating protein degradation. DnaJA4 contains cysteine-rich regions that form zinc finger motifs, contributing to peptide binding domain and overall chaperone function. |
| Product Image Gallery | |
 Western blot analysis of extracts on TM4, Jurkat cells, using DnaJA4 Rabbit Polyclonal Antibody (EAB11270) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemistry of paraffin-embedded Human liver cancer tissue, using DnaJA4 Rabbit Polyclonal Antibody (EAB11270) at 1:50 dilution.
|
