| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF/ICC, FC, ELISA | 36 | Human, Mouse, Monkey |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:100-500 IF/ICC=1:100-500 FC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | c-Jun Mouse Monoclonal Antibody detects endogenous levels of c-Jun protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
c-Jun also known as AP1, JUN is a member of the JUN family. c-Jun plays a crucial role in cellular processes as a key component of the transcription factor AP-1, which is essential for regulating gene expression in response to various stimuli, including growth factors and stress signals. c-Jun′s ability to form both homo- and heterodimers with other members of the Jun and Fos families allows binding to the TPA-response element (TRE) with high affinity, particularly when paired with Fos proteins. c-Jun interaction with Ha-Ras enhances transcriptional activity, highlighting importance in oncogenic signaling pathways. Post-translational modifications, such as phosphorylation, can modulate c-Jun activity and stability. |
| Product Image Gallery | |
 Western blot analysis of extracts on COS7, NIH/3T3 cells, using c-Jun Mouse Monoclonal Antibody (EAB21889) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
Predicted band size: 36 kDa; Observed band size: 43 kDa |
