| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IP, IHC-P, IF/ICC | 41 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IP=1:10-100 IHC-P=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | PKA C-alpha Rabbit Monoclonal Antibody detects endogenous levels of PKA C-alpha protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
PKA C-alpha also known as PKAα cat, PRKACA is one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. PKA C-alpha is a critical component of the cAMP-dependent protein kinase A (PKA) holoenzyme, which plays a pivotal role in mediating diverse cellular responses such as proliferation, ion transport, metabolism regulation, and gene transcription. Structurally, PKA C-alpha consists of a conserved kinase domain essential for catalytic activity, and a regulatory domain that modulates function through binding with cAMP. Post-translational modifications, particularly phosphorylation, are vital for regulating PKA C-alpha′s activity, stability, and interactions with other proteins, thereby fine-tuning the signaling pathways governed.
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| Product Image Gallery | |
 Western blot analysis of extracts on Hela, C6 cells, using PKA C-alpha Rabbit Monoclonal Antibody (EAB21890) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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