| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF/ICC, FC, ELISA | 95 | Human |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:100-500 IF/ICC=1:100-500 FC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | CD22 Mouse Monoclonal Antibody detects endogenous levels of CD22 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
CD22 also designated B-lymphocyte cell adhesion molecule (BLCAM), Siglec-2, Leu-14 is a type I integral membrane glycoprotein that plays a crucial role in regulating B cell signaling and maintaining immune homeostasis. CD22 is predominantly expressed on B cells, where CD22 functions as a negative regulator of B cell receptor signaling, thereby preventing excessive activation and promoting self-tolerance. CD22 is located on the cell membrane and is also found in a cytoplasmic form during early stages of B cell development, making CD22 a valuable marker for acute lymphocytic leukemia. Alternative splicing of the CD22 gene results in two isoforms, CD22α and CD22β, with CD22β being more prevalent. CD22 can also exist as a heterodimer composed of CD22β and the shorter CD22α isoform, highlighting the complexity of CD22 regulation and function in the immune response. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, Jurkat, NIH/3T3, HEK293 cells, using CD22 Mouse Monoclonal Antibody (EAB21909) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
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