www.ebiocell.com
| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 35 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 | |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | FCGR2A/CD32a Rabbit Polyclonal Antibody detects endogenous levels of FCGR2A/CD32a protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
FCGR2A also known as Fc gamma RIIa, CD32a is a member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. CD32 plays a crucial role in the immune response by mediating the clearance of immune complexes through macrophages. This function is vital for maintaining homeostasis and preventing autoimmune reactions, as the binding of IgG to CD32 delivers inhibitory signals to B cells, thereby regulating antibody production. CD32 exists in several isoforms generated by alternative splicing of three distinct genes (A, B, and C), which are designated FcgRIIA, FcgRIIB1, FcgRIIB3, and FcgRIIC. These isoforms are expressed on various immune cells, including monocytes, placental trophoblasts, endothelial cells, and B lymphocytes, with specific isoforms found on neutrophils, highlighting CD32′s diverse roles in immune regulation and response. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, HEK293 cells, using FCGR2A/CD32a Rabbit Polyclonal Antibody (EAB11737) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
|
