| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IHC-P, IF/ICC | 20 | Human |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:100-500 IF/ICC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | CD3γ Rabbit Monoclonal Antibody detects endogenous levels of CD3γ protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
CD3γ also known as CD3G is a crucial component of the T cell receptor (TCR) complex, playing a vital role in T cell activation and signaling. Located on the surface of T cells, CD3γ forms part of a multisubunit complex that transmits signals upon recognition of foreign antigens, thereby initiating an immune response. The structure of CD3γ, which includes a single immunoreceptor tyrosine-based activation motif (ITAM), is essential for its function, as this allows for the recruitment of protein kinases like ZAP-70 and Syk, which are pivotal in propagating the activation signal within the cell. CD3γ presence is not only important for T cell development and function but also serves as a marker for identifying T cells in various immunological studies. |
| Product Image Gallery | |
 Western blot analysis of extracts on Jurkat cells, using CD3γ Rabbit Monoclonal Antibody (EAB21962) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
|
