| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF/ICC, ELISA | 87 | Human, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:5000-20000 IHC-P=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | CUL2 Mouse Monoclonal Antibody detects endogenous levels of CUL2 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
CUL2 also known as Cullin2 is a member of the cullin protein family. CUL2 plays a crucial role in the ubiquitin-proteasome system, particularly in the regulation of protein degradation, which is vital for maintaining cellular homeostasis and controlling various biological processes such as the cell cycle, signal transduction, and responses to stress. CUL2 forms complexes with the von Hippel-Lindau (VHL) tumor suppressor protein and elongin B, creating VBC complexes that are structurally similar to the SCF (Skp1/CUL-1/F-box protein) complex. This interaction facilitates the targeted degradation of hypoxia-inducible factor (HIF), a key regulator of oxygen homeostasis, thereby influencing cellular responses to low oxygen levels. CUL2 modulates the stability of HIF and other substrates, underscoring CUL2′s importance in cancer biology and potential therapeutic interventions. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, K562, HEK293 cells, using CUL2 Mouse Monoclonal Antibody (EAB22359) at 1:10000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
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