| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG2b | Monoclonal | WB, IHC-P, IF/ICC, ELISA | 90 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IF/ICC=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | CUL1 Mouse Monoclonal Antibody detects endogenous levels of CUL1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
CUL1 also known as Cullin1 is the mammalian homolog of yeast Cdc53, is an integral component of the E3 ubiquitin ligase complex designated SCF. CUL1 plays a crucial role in the ubiquitin-proteasome pathway, functioning as a key component of the SCF (Skp1/CUL1/F-box protein) E3 ubiquitin ligase complex, which is essential for the targeted degradation of specific proteins. This process is vital for regulating the cell cycle, as CUL1 mediates the ubiquitination and subsequent degradation of critical cell cycle regulators such as cyclin D, p21, and cyclin E. By controlling the levels of these proteins, CUL1 ensures proper cell cycle progression and prevents uncontrolled cell proliferation, which is a hallmark of cancer. Additionally, CUL1 interacts with various F-box proteins, such as Skp2, to determine substrate specificity, highlighting CUL1′s importance in maintaining cellular homeostasis and responding to cellular signals. |
| Product Image Gallery | |
 Western blot analysis of extracts on Jurkat, NIH/3T3 cells, using CUL1 Mouse Monoclonal Antibody (EAB22143) at 1:3000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
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