| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IHC-P, IF/ICC | 19 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IHC-P=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Cofilin Rabbit Monoclonal Antibody detects endogenous levels of Cofilin protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Cofilin also known as CFL is a critical actin-binding protein that plays a crucial role in regulating actin dynamics, which is essential for various cellular processes, including motility, division, and apoptosis. Cofilin is primarily located in the cytoplasm, where Cofilin interacts with filamentous actin (F-actin) to promote actin filament disassembly, facilitating rapid cytoskeleton remodeling. Cofilin′s ability to bind F-actin is vital for maintaining cellular integrity and function, allowing cells to respond to environmental cues and adapt shape and movement accordingly. Additionally, Cofilin′s activity is regulated by phosphorylation; when phosphorylated by LIM kinase 1, Cofilin becomes inactive and unable to bind F-actin, highlighting the importance of post-translational modifications in controlling function. |
| Product Image Gallery | |
 Western blot analysis of extracts on A549, HEK293, NIH/3T3, C6 cells, using Cofilin Rabbit Monoclonal Antibody (EAB21926) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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