| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IHC-P, IF/ICC | 105 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IP=1:10-100 IHC-P=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | α-Actinin 4 Rabbit Monoclonal Antibody detects endogenous levels of α-Actinin 4 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
α-Actinin 4 also known as Actinin alpha 4, ACTN4 is a member of the spectrin gene family, which encodes a diverse group of cytoskeletal proteins, including spectrins, dystrophins, and α-actinins. α-actinin 4 is primarily located in the cytoskeleton of muscle and non-muscle cells, such as skeletal, cardiac, and smooth muscle cells, where α-actinin 4 plays a vital role in maintaining cellular structure and integrity. The unique structure of α-actinin 4, which includes one actin-binding domain, two calponin-homology domains, two EF-hand domains, and four spectrin repeats, allows α-actinin 4 to function effectively as a bundling protein that cross-links filamentous actin (F-actin), anchoring F-actin to various intracellular structures. This anchoring is essential for cellular processes such as muscle contraction and cell signaling. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, HEK293, Raw264.7 cells, using α-Actinin 4 Rabbit Monoclonal Antibody (EAB21860) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue, using α-Actinin 4 Rabbit Monoclonal Antibody (EAB21860) at 1:100 dilution.
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