| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IHC-P, IF/ICC, FC | 71 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IP=1:10-100 IHC-P=1:50-200 IF/ICC=1:50-200 FC=1:10-100 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | MEKK3 Rabbit Monoclonal Antibody detects endogenous levels of MEKK3 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
MEKK3(MEK kinase 3) also known as MAP3K3 is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. MEKK3 plays a crucial role in mitogen-activated protein (MAP) kinase signaling pathways, which are essential for cellular responses to various extracellular stimuli, including growth factors. Specifically, MEKK3 is involved in phosphorylation and activation of downstream MAP kinases, such as ERK, JNK, and p38, which are pivotal for regulating processes like cell proliferation, differentiation, and survival. Precise regulation of these pathways is vital for maintaining normal cellular functions and preventing diseases such as cancer, where aberrant signaling can lead to uncontrolled cell growth. |
| Product Image Gallery | |
 Western blot analysis of extracts on A431, 293T cells, using MEKK3 Rabbit Monoclonal Antibody (EAB21841) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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