| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IF/ICC | 69 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IF/ICC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | APC8/Cdc23 Rabbit Monoclonal Antibody detects endogenous levels of APC8/Cdc23 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
APC8 also known as Cdc23 is a component of the tetratricopeptide repeat (TPR) APC/C sub-complex that also includes APC3 (CDC27) and APC6 (CDC16). APC8 plays a crucial role in the anaphase-promoting complex (APC), which is essential for proper cell cycle progression. APC8 functions as a component of APC that facilitates ubiquitination of mitotic cyclins and other regulatory proteins, promoting sister chromatid separation during metaphase to anaphase transition in mitosis. This process ensures accurate chromosome segregation, which maintains genomic stability and prevents aneuploidy, a condition often associated with cancer. APC8 contains nine tetratricopeptide repeat (TPR) units, which mediate protein-protein interactions, enabling APC to function effectively. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, Jurkat cells, using APC8/Cdc23 Rabbit Monoclonal Antibody (EAB21831) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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