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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IHC-P, FC | 17 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:50-200 FC=1:10-100 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | HSP20 Rabbit Monoclonal Antibody detects endogenous levels of HSP20 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
HSP20(Heat Shock Protein 20) also known as HSPB6 is a ubiquitously expressed molecular chaperone, with higher levels found in skeletal, cardiac, and smooth muscle. HSP20 plays a cardioprotective role in response to injury through cardiac β-adrenergic signaling, which induces increased HSP20 protein expression and phosphorylation at Ser16 by PKA. Phosphorylation of HSP20 at Ser16 protects cells from undergoing apoptosis and mediates HSP20 interaction with the cytoskeleton. Constitutive phosphorylation of HSP20 at Ser16 in mice leads to fibrosis and heart failure through interleukin-6 (IL-6) and STAT3 signaling. |
| Product Image Gallery | |
 Western blot analysis of extracts on Human skeletal muscle, Mouse skeletal muscle cells, using HSP20 Rabbit Monoclonal Antibody (EAB21828) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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