| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IHC-P, IF/ICC | 54 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IP=1:10-100 IHC-P=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | FoxO4 Rabbit Monoclonal Antibody detects endogenous levels of FoxO4 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
FoxO4 also known as AFX1 is a member of the O class of winged helix/forkhead transcription factor family. FoxO4 plays a crucial role in regulating cellular processes such as apoptosis, cell cycle progression, and oxidative stress response, making FoxO4 vital for maintaining cellular homeostasis and preventing tumorigenesis. FoxO4 is predominantly located in the nucleus, where FoxO4 functions as a transcription factor, binding to specific DNA sequences to modulate gene expression. FoxO4 nuclear localization is tightly regulated by post-translational modifications, particularly phosphorylation by the serine/threonine kinase Akt1, which sequesters FoxO4 in the cytosol, thereby inhibiting transcriptional activity. Dysregulation of FoxO4 has been implicated in various cancers, including acute lymphocytic leukemia and alveolar rhabdomyosarcoma, highlighting FoxO4′s importance as a potential therapeutic target. |
| Product Image Gallery | |
 Western blot analysis of extracts on Jurkat, K562, C6 cells, using FoxO4 Rabbit Monoclonal Antibody (EAB21815) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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