| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IHC-P, IF/ICC, FC | 49 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IP=1:10-100 IHC-P=1:50-200 IF/ICC=1:50-200 FC=1:10-100 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | RUNX1/RUNX2/RUNX3 Rabbit Monoclonal Antibody detects endogenous levels of RUNX1/RUNX2/RUNX3 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
The mammalian Runt-related transcription factor (RUNX) family comprises three members, RUNX1, RUNX2 and RUNX3. RUNX family members are DNA-binding proteins that regulate the expression of genes involved in cellular differentiation and cell cycle progression. RUNX1 plays a critical role in hematopoiesis, the process of blood cell formation, and is essential for proper development of various blood cell lineages. RUNX1 regulates the expression of genes involved in cellular differentiation and cell cycle progression, making RUNX1 a key player in maintaining normal hematopoietic function. RUNX1 is frequently implicated in human leukemia due to chromosomal translocations that disrupt DNA-binding domain, leading to aberrant gene expression and oncogenesis. RUNX2 plays a critical role in skeletal development and mineralization, functioning as a transcription factor that regulates the expression of genes essential for osteoblast differentiation and bone formation. RUNX3 plays a crucial role in regulating gene expression related to cellular differentiation and cell cycle progression. RUNX3 is predominantly expressed in hematopoietic cells, including myeloid and B-cell lines, as well as in the spleen. |
| Product Image Gallery | |
 Western blot analysis of extracts on Jurkat cells, using RUNX1/RUNX2/RUNX3 Rabbit Monoclonal Antibody (EAB21360) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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