| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IHC-P, IF/ICC | 57 | Human, Mouse, Rat, Dog |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IP=1:10-100 IHC-P=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | RUNX2 Rabbit Monoclonal Antibody detects endogenous levels of RUNX2 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
RUNX2 also known as AML-3, PEBP2αA, CBFA1, Osf2 is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. RUNX2 plays a critical role in skeletal development and mineralization, functioning as a transcription factor that regulates the expression of genes essential for osteoblast differentiation and bone formation. RUNX2 stimulates the differentiation of mesenchymal stem cells into osteoblasts for proper skeletal development and promotes chondrocyte hypertrophy, which is crucial for endochondral ossification. RUNX2 drives endothelial cell migration and vascular invasion during bone development, highlighting RUNX2′s importance in both skeletal and vascular systems. |
| Product Image Gallery | |
 Western blot analysis of extracts on MDA-MB-231, NIH/3T3 cells, using RUNX2 Rabbit Monoclonal Antibody (EAB21796) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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