| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IHC-P, IF/ICC | 132 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IP=1:10-100 IHC-P=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | IRS-1 Rabbit Monoclonal Antibody detects endogenous levels of IRS-1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
IRS-1(Insulin receptor substrate 1) is one of the major substrates of the insulin receptor kinase. IRS-1 plays a crucial role in insulin signaling as a major substrate of the insulin receptor, being phosphorylated in response to insulin, insulin-like growth factor 1 (IGF-1), and interleukin 4 (IL-4) stimulation. Phosphorylation occurs on serine, threonine, and tyrosine residues across various tissues, with casein kinase II being a key serine/threonine kinase that mediates a significant portion of insulin-stimulated phosphorylation of IRS-1. Notably, threonine 502 has been identified as the primary phosphorylation site for casein kinase II in rat IRS-1, while serine 99 serves as an additional phosphorylation site. Phosphorylation of IRS-1 by casein kinase II is an integral part of the intracellular insulin signaling cascade, influencing metabolic responses to insulin. |
| Product Image Gallery | |
 Western blot analysis of extracts on A549, C2C12, C6 cells, using IRS-1 Rabbit Polyclonal Antibody (EAB21974) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 132 kDa; Observed band size: 160-185 kDa
 Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue, using IRS-1 Rabbit Monoclonal Antibody (EAB21794) at 1:100 dilution.
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