| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IHC-P | 28 | Human, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | 14-3-3 sigma Rabbit Monoclonal Antibody detects endogenous levels of 14-3-3 sigma protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
14-3-3 sigma also known as SFN, stratifin, HME1, YWHAS plays a crucial role in cellular signaling and regulation, particularly in cancer biology. 14-3-3 sigma is predominantly expressed in stratified squamous keratinizing epithelium and functions as a secreted adaptor protein that interacts with various target proteins, including Keratin 17. 14-3-3 sigma binding to Keratin 17 enhances the Akt/mTOR signaling pathway, promoting protein synthesis and cell growth, which are vital processes in tumor development and progression. 14-3-3 sigma positively regulates tumor suppressor p53, influencing cell cycle checkpoints and apoptosis. This modulation of key pathways highlights 14-3-3 sigma′s importance in maintaining cellular homeostasis and potential as a therapeutic target in cancer treatment. |
| Product Image Gallery | |
 Western blot analysis of extracts on A431 cells, using 14-3-3 sigma Rabbit Monoclonal Antibody (EAB21791) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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