| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IHC-P, IF/ICC, FC | 50 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-200 IF/ICC=1:50-200 FC=1:10-100 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | AP2M1 Rabbit Monoclonal Antibody detects endogenous levels of AP2M1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
AP2M1 also AP2u1 is a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. AP2M1 plays a significant role in clathrin-mediated endocytosis, a vital cellular process for internalizing nutrients and signaling molecules. AP2M1 is primarily located at the plasma membrane and is essential for linking clathrin to specific receptors in coated vesicles, facilitating the transport of ligand-receptor complexes from the cell surface to intracellular compartments. Proper functioning of AP2M1 is critical for maintaining cellular homeostasis and regulating various signaling pathways, as disruptions in this process can lead to impaired endocytosis and contribute to various diseases, including cancer and neurodegenerative disorders. |
| Product Image Gallery | |
 Western blot analysis of extracts on PC3M cells, using AP2M1 Rabbit Monoclonal Antibody (EAB21784) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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