| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IF/ICC | 34 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IP=1:10-100 IF/ICC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | CDK4 Rabbit Monoclonal Antibody detects endogenous levels of CDK4 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
CDK4 is a vital member of the cyclin-dependent kinase family, playing a crucial role in regulating cell cycle progression by partnering with D-type cyclins to control cell growth during the G1 phase. The function of CDK4 is essential for the phosphorylation of key substrates that drive the cell cycle forward, making CDK4 a significant focus in cancer research and studies of cellular proliferation. The activity of CDK4 is modulated through interaction with cyclins, which enhance kinase activity, and inhibitors like p16, which suppress function. Additionally, the activation of CDK4-cyclin complexes involves phosphorylation by Cdk-activating proteins (CAK), highlighting the sophisticated regulatory mechanisms governing cell cycle progression. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, MCF7, K562 cells, using CDK4 Rabbit Monoclonal Antibody (EAB21774) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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