| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IHC-P, IF/ICC | 39 | Human, Mouse |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IP=1:10-100 IHC-P=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Oct-4 Rabbit Monoclonal Antibody detects endogenous levels of Oct-4 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Oct-4 also known as Oct-3/4, POU5F1 is referred to as octamer-binding transcription factor-3 (Oct-3 or Otf-3) and octamer-binding transcription factor-4 (Oct-4 or Otf-4), respectively. Oct-4 plays a crucial role in regulating embryonic stem cell populations by influencing lineage commitment and maintaining pluripotency. This transcription factor is essential for sustaining stem-cell self-renewal and guiding differentiation pathways, which are vital for proper embryonic development. Oct-4 activates the UTF1 gene, promoting rapid proliferation and tumorigenic properties in embryonic stem cells. In humans, two isoforms of Oct-4 are involved in maintaining the undifferentiated state of embryonic stem cells, while pseudogenes located on chromosomes 10 and 8 have been shown to be transcribed in certain cancer cell lines and tissues, highlighting Oct-4′s significance in both normal development and cancer biology. |
| Product Image Gallery | |
 Western blot analysis of extracts on F9, NCCIT cells, using Oct-4 Rabbit Monoclonal Antibody (EAB21771) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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