| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IHC-P, IF/ICC, FC | 21 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-200 IF/ICC=1:50-200 FC=1:10-100 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | ARF1 Rabbit Monoclonal Antibody detects endogenous levels of ARF1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
ARF1(ADP-ribosylation factor 1) is a member of the human ARF gene family. ARF1 plays a crucial role in cellular processes such as vesicular transport and membrane trafficking, which are vital for maintaining organelle integrity and cellular homeostasis. Located primarily at the Golgi apparatus, ARF1 is involved in the recruitment of coatomer protein complex I (COPI) to membranes, facilitating the formation of transport vesicles. This recruitment is regulated by the binding and hydrolysis of guanosine triphosphate (GTP), highlighting ARF1′s importance in the dynamic regulation of membrane traffic. ARF1′s ability to interact with specific membrane phospholipids further underscores its significance in cellular signaling and transport mechanisms. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, THP-1 cells, using ARF1 Rabbit Monoclonal Antibody (EAB21762) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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