| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IF/ICC | 51 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | RARα Monoclonal Antibody detects endogenous levels of RARα protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
RARα also known as RARalpha, RARA is a crucial nuclear transcription factor located within the cell nucleus, where RARα mediates the effects of retinoic acid, a vital metabolite of vitamin A essential for regulating gene expression involved in cell differentiation, proliferation, and apoptosis. The proper functioning of RARα is imperative for vertebrate development and maintaining tissue homeostasis, and RARα dysregulation is closely associated with various diseases, notably acute promyelocytic leukemia (APL). In APL, a chromosomal translocation fuses the RARα gene with the PML gene, resulting in the oncogenic PML/RARα fusion protein that disrupts normal apoptotic pathways and halts myeloid differentiation. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, PC12, SKBR3 cells, using RARα Rabbit Monoclonal Antibody (EAB21658) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
|
