| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, FC | 54 | Human, Zebrafish |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 FC=1:10-100 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | BPI Rabbit Monoclonal Antibody detects endogenous levels of BPI protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
BPI is part of a functionally related protein family that includes lipopolysaccharide binding protein, phospholipid transfer protein, and cholesteryl ester transfer protein, highlighting BPI′s importance in the broader context of immune defense mechanisms. BPI plays a crucial role in the immune response by acting as an antibacterial and endotoxin-neutralizing agent, primarily found in the granules of polymorphonuclear leukocytes, such as neutrophils. The human BPI gene, which spans 31.5 kb and is located on chromosome 20q11.23-q12, consists of 15 exons and encodes a 456 amino acid protein. BPI is strategically localized to the cell surface of epithelial cells, which serve as the first line of defense against bacterial invasion. Such localization enables BPI to block endotoxin-mediated signaling, thereby safeguarding mucosal surfaces from the harmful effects of gram-negative bacteria and their endotoxins. |
| Product Image Gallery | |
 Western blot analysis of extracts on THP-1 cells, using BPI Rabbit Monoclonal Antibody (EAB21726) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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