| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP | 112 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IP=1:10-100 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | NEDD4L Rabbit Monoclonal Antibody detects endogenous levels of NEDD4L protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
NEDD4L also konwn as NEDD2 is a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. NEDD4L functions as an E3 ubiquitin-protein ligase, facilitating the transfer of ubiquitin from an E2 ubiquitin-conjugating enzyme to specific substrate proteins, which are then marked for proteasomal degradation. By ubiquitinating key proteins such as Smad2 and TGFβ RII, NEDD4L inhibits the TGFβ signaling pathway, a critical regulator of cell growth and differentiation. NEDD4L′s ability to modulate these pathways highlights its importance in various cellular processes and potential implications in diseases where TGFβ signaling is dysregulated. NEDD4L is expressed as eight isoforms due to alternative splicing, contributing to functional diversity and regulatory mechanisms within different tissues, particularly in the pancreas, prostate, and kidney where expression is highest. |
| Product Image Gallery | |
 Western blot analysis of extracts on Jurkat cells, using NEDD4L Rabbit Monoclonal Antibody (EAB21711) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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