| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IHC-P, IF/ICC | 150 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IP=1:10-100 IHC-P=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | PLCγ1 Rabbit Monoclonal Antibody detects endogenous levels of PLCγ1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Phospholipase C-gamma 1 (PLCγ1) is an isozyme of the phosphoinositide-specific PLC family. PLCγ1 plays a pivotal role in receptor-mediated signal transduction by generating second messengers inositol 1,4,5-triphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate, which are essential for various cellular processes. PLCγ1 is predominantly located in the cytoplasm and is widely distributed in tissues such as bronchiolar epithelium, type I and II pneumocytes, and fibroblasts of the interstitial tissue, highlighting its importance in respiratory function and cellular signaling. The interaction of PLCγ1 with actin-regulatory protein Villin, which is tyrosine phosphorylated, further underscores its significance; Villin modulates PLCγ1 activity by altering binding affinity to phosphatidylinositol 4,5-bisphosphate, thereby influencing cellular responses. |
| Product Image Gallery | |
 Western blot analysis of extracts on MCF7, SH-SY5Y cells, using PLCγ1 Rabbit Monoclonal Antibody (EAB21694) at 1:2000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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