| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IF/ICC | 68 | Human, Mouse |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IP=1:10-100 IF/ICC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-SHP-2 (Tyr542) Rabbit Monoclonal Antibody detects endogenous levels of SHP-2 protein only when phosphorylated at Tyr542. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
SHP-2 also known as SH-PTP2, PTPN11 is a member of the protein tyrosine phosphatase (PTP) family. SHP-2 plays a crucial role in cellular signaling by regulating the phosphorylation state of tyrosine residues on proteins, which is essential for controlling various cellular processes such as growth, differentiation, and metabolism. Located primarily in the cytoplasm, SHP-2 interacts with a variety of signaling molecules, including growth factor receptors and other phosphotyrosine-containing proteins, thereby influencing pathways that are vital for cellular responses to external stimuli. The importance of SHP-2 is underscored by its involvement in several diseases, including cancer, where dysregulation of its activity can lead to aberrant signaling and tumor progression. With its ability to modulate key signaling pathways, SHP-2 serves as a significant target for therapeutic intervention and research into cellular mechanisms. |
| Product Image Gallery | |
 Western blot analysis of extracts on NIH/3T3 cells, using Phospho-SHP-2 (Tyr542) Rabbit Monoclonal Antibody (EAB21705) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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