| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP | 27 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IP=1:10-100 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-HSP27 (Ser82) Rabbit Monoclonal Antibody detects endogenous levels of HSP27 only when phosphorylated at Ser82. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
HSP27 is a member of the heat shock protein family, functioning primarily as a molecular chaperone that assists in protein folding and protects cells from stress-induced damage. HSP27 is predominantly located in the cytoplasm but translocates to the nucleus under stress conditions, highlighting its role in cellular response mechanisms. HSP27′s ability to relocate allows for the regulation of various cellular processes, including apoptosis and cell survival, particularly in response to heat shock, cytokines, and hormonal signals. Notably, HSP27 undergoes extensive post-translational modifications, including phosphorylation, which enhances chaperone activity and is critical for protective functions during cellular stress. HSP27′s regulation by hormones like estrogen suggests a potential link to tumorigenesis in breast cancer, making HSP27 a valuable target for understanding cancer biology and developing therapeutic strategies. |
| Product Image Gallery | |
 Western blot analysis of extracts on Human skeletal muscle tissue, using Phospho-HSP27 (Ser82) Rabbit Monoclonal Antibody (EAB21913) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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