| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IHC-P, IF/ICC | 94 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IHC-P=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Atg9A Rabbit Monoclonal Antibody detects endogenous levels of Atg9A protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Atg9A also known as APG9L1 is a crucial multi-pass membrane protein consisting of 839 amino acids that plays a significant role in the autophagy process, which is essential for cellular homeostasis and the degradation of damaged organelles and proteins. Atg9A is primarily localized to the pre-autophagosomal structure (PAS), where Atg9A is involved in the formation of isolation membranes that eventually wrap around cytoplasmic components to create double-membrane autophagosomes. These autophagosomes then fuse with lysosomes for degradation, highlighting Atg9A′s importance in maintaining cellular health, especially during nutrient deprivation. Atg9A cycling between the Golgi apparatus and endosomes, along with Atg9A interaction with the autophagosome-specific marker LC3, underscores Atg9a′s critical function in starvation-induced autophagosome formation. |
| Product Image Gallery | |
 Western blot analysis of extracts on HepG2, Rat brain cells, using Atg9A Rabbit Monoclonal Antibody (EAB21649) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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