| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IHC-P, IF/ICC | 17 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IP=1:10-100 IHC-P=1:50-200 IF/ICC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Myoglobin Rabbit Monoclonal Antibody detects endogenous levels of Myoglobin protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Myoglobin(MB) is a member of the globin superfamily and is predominantly expressed in skeletal and cardiac muscles. Myoglobin is a crucial cytosolic oxygen-binding protein primarily located in skeletal and cardiac muscle, where myoglobin plays a vital role in oxygen storage and diffusion within myocytes. Myoglobin is essential for maintaining mitochondrial respiration during intense and prolonged muscle activity, facilitating oxygen transport from erythrocytes to mitochondria, which is critical for energy production. Myoglobin′s structure, characterized by a heme group, allows effective oxygen binding and release, making myoglobin indispensable for muscle function and endurance. Additionally, myoglobin′s genomic structure is conserved across various species, featuring a putative polyadenylation signal and a polypyrimidine-rich region, with human myoglobin being encoded by a single gene that has also been identified in human smooth muscle. |
| Product Image Gallery | |
 Western blot analysis of extracts on Human skeletal muscle cells, using Myoglobin Rabbit Monoclonal Antibody (EAB21632) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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